Composite

Part:BBa_K3095011

Designed by: Dora Bonadio, Rafael Zuccarelli, Jung Hun Park, Alice Kei Endo   Group: iGEM19_USP-Brazil   (2019-10-16)


Repressors feedback loop

Uses Tet and Lac repressors to create a control feedback loop.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2790
    Illegal NotI site found at 1333
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4920
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2882
    Illegal BsaI site found at 3757
    Illegal BsaI.rc site found at 2855
    Illegal BsaI.rc site found at 3730


[edit]
Categories
Parameters
None